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People at risk include those who: Currently inject drugs Injected drugs in the past, even if it was once or many years ago Have HIV or AIDS Have abnormal liver tests or liver disease Received donated blood or organs before 1992 Have been exposed to blood on the job through a needlestick or injury with a sharp object Are getting long-term hemodialysis

The specific steps were as follows: First, BAM files generated from the mapping of tobacco leaf samples to the reference genome were sorted using SAMtools (v1.21) ( Subsequently, the SplitNCigarReads function in GATKwas applied to split reads into exon segments (removing Ns while maintaining grouping information) and hard-clip any sequences overhanging into intronic regions ( Identification of sQTL ASEs were identified using LeafCutter ( Results Extensive transcriptome diversity and complexity in tobacco In this study, a total of 207,689 transcripts were identified from 599 transcriptome datasets derived from 13 different tissues

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